Pock forming ability of fowl pox virus isolated from layer chicken and its adaptation in chicken embryo fibroblast cell culture

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Pock forming ability of fowl pox virus isolated from layer chicken and its adaptation in chicken embryo fibroblast cell culture.

AIM The objective of the present study was to examine pock forming ability of field strain and vaccine strain of fowl pox virus (FPV) in chorioallantoic membrane (CAM) of embryonated chicken eggs and its adaptation in chicken embryo fibroblast (CEF) cell culture. MATERIALS AND METHODS Dry scabs were collected from 25 affected birds in glycerin-saline and preserved at 4°C until processed. Viru...

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Molecular diagnosis and adaptation of highly virulent infectious bursal disease virus on chicken embryo fibroblast cell

Materials and Methods Infectious bursal disease (IBD) is a highly contagious disease of young chicken caused by infectious bursal disease virus (IBDV), characterized by immunosuppression and mortality generally at 3 to 6 weeks of age [1]. It is economically important to the poultry industry worldwide, due to increased susceptibility to other diseases and negative interference with effective vac...

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Evaluation and Optimization of Chick Embryo Fibroblasts for Production of a Fowl Pox Vaccine Based on Cell Culture

Abstract Background and Aims: For more than half a century, the production of fowl pox vaccine at Razi Vaccine and Serum Research Institute, has been carried out by injection method in the chick chorioallantoic membrane (CAM) and the vaccine has a favorable and effective in poultry flocks and has provided a complete satisfaction to the poultry flocks owner. Fowl pox vaccine is also manufactured...

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telomerase activity in chicken embryo fibroblast cell cultures infected with marek's disease virus

background : telomerase is a ribonucleoprotein, which adds telomeric repeats onto the 3’ end of existing telomers at the end of chromosomes in eukaryotes. one hypothesis states that telomere length may function as a mitotic clock, therefore expression of telomerase activity in cancer cells may be a necessary and essential step for tumor development and progression. methods : the detectability o...

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Transcriptome Analysis of Chicken Embryo Fibroblast Cell Infected with Marek’s Disease Virus of GX0101∆LTR

Marek’s disease (MD), a lymphoproliferative disorder of chickens caused by the MD virus (MDV), is economically significant. The resistance/susceptibility to MD is controlled by host genetics. The host response to different virus strains varies. The pathogenicity of REV-LTR deleted GX0101∆LTR MDV has been previously reported. However, the precise molecular mechanism of the response of chickens t...

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ژورنال

عنوان ژورنال: Veterinary World

سال: 2015

ISSN: 0972-8988,2231-0916

DOI: 10.14202/vetworld.2015.245-250